recomLine Bordetella pertussis IgG
recomLine Bordetella pertussis IgA
Strip-Immunoassay with antigens produced by recombinant techniques for the detection of IgG or IgA antibodies against Bordetella pertussis
Bordetella pertussis is the main agent of the „whooping cough“, a disease often lasting for weeks til months. The passive immunoprotection transferred from mother to fetus is usually incomplete or inexistent. Therefore especially infants are imperiled by apnea (stop breathing) which can lead to death. Mild courses can be caused by related agents i.e. B. parapertussis. Several vaccinations are recommended especially for infants to provide at least some immunoprotection.Besides the clinical diagnostics (long persisting cough with staccato cough attacks) the serological diagnostics is applied to determine the status of immunity (possibly after vaccination) and an acute infection. The new antigen PT-100 is used to standardize the recomLine Bordetella pertussis according to the WHO-standard and the guidelines of the German Robert-Koch-Institute. PT-100 detects an antibody titer >= 100 WHO units. A reactive PT-100 band corresponds with high probability to an acute infection when clinical symptoms appear (considering the vaccination managment).
The recomLine Bordetella pertussis IgG, IgA uses the antigens pertussis toxin (PT), specific for B. pertussis, and filamentous haemagglutinin (FHA) which all representatives of Bordetella species have in common.
After screening by ELISA the recomLine Bordetella pertussis IgG, IgA can be used for clarification of borderline and positive ELISA-results and for the determination of the actual immune status.
Product advantages
- Recombinant antigens
- High sensitivity and specificity
- Easy and clear interpretation due to easy to read bands
- PT-100 standardized by the WHO-Standard - hint for an acute infection
- Easy test procedure; automation possible
- Easy and objective evaluation and documentation by recomScan software
- Test procedure and reagents identical in all MIKROGEN strip tests - reagents exchangeable
- Separate detection of IgG and IgA antibodies - conjugate control on each strip
- Safe evaluation due to strip specific cutoff control
- CE label: The recomLine Bordetella tests meet the high standard of the EC directive 98/79/EC on in vitro diagnostic medical devices
Testprinciple and procedure
1st Incubation
A test strip loaded with Bordetella antigens is incubated with diluted serum or plasma in a dish for 1 hour.
wash 3 times
2nd Incubation
Peroxidase conjugated anti-human antibodies (IgG specific) are added. Incubate for 45 minutes.
wash 3 times
Color reaction
8 minutes after addition of the coloring solution, insoluble colored bands develop at the sites on the test strips occupied by antibodies.
Evaluation
Sensitivity and specificity
Samples from patients with acute pertussis infection
Samples strips from patients with acute pertussis infection
