recomWell Borrelia canis IgG
recomWell Borrelia canis IgM
Enzyme immunoassay with recombinant antigens for the detection of IgG or IgM antibodies against Borrelia burgdorferi in dog
The bacterium Borrelia burgdorferi, a spirochete, is responsible for Lyme borreliosis. This disease is transmitted to dogs by ticks. Lyme borreliosis is a multisystemic disease with diverse manifestations which make clinical diagnosis difficult. Direct detection of the pathogen is also problematic. Therefore, in routine diagnosis, the serological detection of Borrelia-specific antibodies represents the easiest and safest method for the detection of a Borrelia infection.
Enzyme immunoassays or immunofluorescence tests are mainly used for screening in veterinary medicine. In the recomWell Borrelia canis tests genetically engineered antigens are used. In contrast to ELISA with lysate antigen from Borrelia burgdorferi cells, the recombinant antigens can be weighted differently when coating, so that otherwise under-represented antigens can be offered in adequate amount. This applies especially to the OspC antigen. In addition, the genetical engineering allows the simultaneous use of antigens from different genospecies.
Therefore, the recomWell Borrelia canis tests define a new standard in veterinary diagnosis. It is only with the recomWell Borrelia canis tests, that antibodies against all three genospecies
- Borrelia burgdorferi sensu stricto,
- Borrelia garinii
- Borrelia afzelii
can be safely detected in one test run.
Product advantages
- Recombinant antigens, therefore
- High sensitivity and specificity
- Optimum presentation without cross-reacting Borrelia proteins
- Excellent discrimination between negative and positive results
- Immunodominant antigens of the three genospecies: B. burgdorferi sensu stricto, B. garinii and B. afzelii
- Use of different immunodominant antigens for the early and late phase:
- IgM: OspC, p41/internal, VlsE
- IgG: p100, OspC, VlsE, p18
- Separate detection of IgG and IgM antibodies
- Identical procedure for IgG and IgM determination
- No RF absorbance necessary in the case of IgM determination
- Easy to quantify
- Standardised serum-liquor-analysis available - CE certified instructions for use
- Easy test procedure; automation possible
- Test procedure and reagents identical in all MIKROGEN ELISA - reagents exchangeable
- Break-a-parts: single sample examination possible
Testprinciple and procedure
Indirect sandwich test.
Recombinant antigens are bound to the solid phase.
1st Incubation
Add patient samples diluted 1:101 (sample: 10 µl of serum or plasma), incubate for 1 h at 37 °C.
Wash 4 times
2nd Incubation
Add peroxidase conjugated anti-human IgG or IgM antibodies (conjugate), incubate for 30 min at 37 °C.
Wash 4 times
Color reaction
Add ready-to-use TMB solution and incubate for 30 min at room temperature. Stop the substrate reaction with H3PO4 and measure the extinction at 450 nm.
Evaluation
A selection of dogs from Southern Germany*, which are employed in hunt, shows the following results with recomWell Borrelia canis:
Samples from Hounds
*Kindly provided by the Academy for Forestry, Rottenburg, Germany
Specificity
Samples from 31 puppies and 21 experimental dogs prior to infection were used for the determination of the specificity
