Fluorescence based particle immunoassay using recombinant antigens for the detection of IgG, IgA or IgM antibodies against Y. enterocolitica and Y. pseudotuberculosis. By determination of species specific IgG antibodies, a differentiation between Y. enterocolitica and Y. pseudotuberculosis is possible.

The enteropathogenic Yersinia species, Yersinia enterocolitica and Yersinia pseudotuberculosis, have a global distribution. Transmissions occur orally either in food (especially meat) or in contaminated water. Typical symptoms of an acute Y. enterocolitica infection are watery, sometimes bloody diarrhoea with abdominal pain, vomiting and fever. A Y.  pseudotuberculosis infection is difficult to distinguish from appendicitis, it is also referred to as “pseudoappendicitis”. Post infectious complications such as reactive arthritis, erythema  nodosum and other rheumatic diseases can occur, especially with HLA-B27 carriers. High and persistent IgA titres against Yersinia antigens are characteristic of these patients.

The modern Luminex^® multiplex technology integrates the advantages from ELISA and strip assays of antibodies against individual antigens. Genetically engineered virulence factors and adhesins are used for the multiplex recomBead Yersinia 2.0 test systems. These proteins are expressed only by Yersinia strains that are pathogenic for humans. The recomBead  Yersinia 2.0 test systems allow the serologic differentiation of species, as well as the determination of long past Yersinia infections and are thus ideally suited for identification of  Yersinia-induced immunopathological complications and chronic yersiniosis. Detection of IgG and IgA antibodies can be a very useful diagnostic tool if Yersinia-induced arthritis is suspected.

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