Strip-Immunoassay with antigens produced by recombinant techniques for the detection of IgG or IgM antibodies against Treponema pallidum

Treponema pallidum subsp. pallidum is the causative agent of Syphilis, a chronic disease occuring only in humans. The disease progresses in several stages. After a short propagation period at the site of infection (primary stage) the hematogenic spreading of the pathogen throughout the entire organism follows (secondary stage). After the decline of acute symptoms Treponema pallidum can persist for years or lifetime in the human organism (stage of latency) and  then can cause late complications (tertiary Syphilis and neurosyphilis). During pregnancy the pathogen can be transmitted to the fetus (Syphilis connata). An untreated syphilis can decisively affect the course of pregnancy and can lead to spontaneous abortion, stillborn or premature birth.

The serologic diagnosis of syphilis intends the use of a sensitive antibody screening assay (TPHA/TPPA, ELISA) in the first step. In the case of equivocal or positive results in the screening test a confirmation assay is performed in the second step to assure the specificity (FTA-ABS, immunoblot). Here the immunoblot plays a very important role, as only with this technique the determination of antibody production against essential Treponema pallidum antigens is possible. At the same time the detection of different antibody classes is possible. With the use of recombinant antigens the recomLine Treponema IgG, IgM meets all these requirements perfectly.

Commercial product